itaq universal sybr green (Bio-Rad)
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itaq universal sybr green
Itaq Universal Sybr Green, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/itaq+universal+sybr+green/iTaq+Universal+SYBR+Green+One-Step+Kit/pmc13084690-101-14-20
Average 96 stars, based on 1321 article reviews
Itaq Universal Sybr Green, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/itaq+universal+sybr+green/iTaq+Universal+SYBR+Green+One-Step+Kit/pmc13084690-101-14-20
Average 96 stars, based on 1321 article reviews
itaq universal sybr green - by Bioz Stars,
2026-09
96/100 stars
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Expressing:Article Title: Skeletons in the closet: The importance of actin in alphavirus replication Article Snippet: To measure Wolbachia titer, six-day-old mated female flies were snap-frozen in liquid nitrogen and stored at -80°C until genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen). qPCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad) on the CFX Opus 96 (Bio-Rad). .. To measure rhoGAP18B expression, six-day-old mated female flies were snap-frozen in liquid nitrogen and stored at -80°C until RNA was extracted using the RNeasy Mini Kit (Qiagen). qRT-PCR was performed using the Quantitative RT-PCR:Article Title: Skeletons in the closet: The importance of actin in alphavirus replication Article Snippet: To measure Wolbachia titer, six-day-old mated female flies were snap-frozen in liquid nitrogen and stored at -80°C until genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen). qPCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad) on the CFX Opus 96 (Bio-Rad). .. To measure rhoGAP18B expression, six-day-old mated female flies were snap-frozen in liquid nitrogen and stored at -80°C until RNA was extracted using the RNeasy Mini Kit (Qiagen). qRT-PCR was performed using the Article Title: Local translation drives glioblastoma heterogeneity and tumor invasion Article Snippet: RNA quantity and integrity were assessed using an Agilent 4200 TapeStation with a High Sensitivity RNA ScreenTape (Agilent Technologies). .. Quantitative RT–PCR was performed using the Article Title: Extracts from the Edible Mushroom Sparassis crispa : Nematicidal, Antimicrobial, and Antiviral Properties Supporting Its Functional Food Potential. Article Snippet: The RNA of CVB3 and DNA of HSV-1 was isolated using QIAamp Viral RNA Mini Kit (Cat.: 52904 QIAGEN GmbH, Hilden, Germany) and QIAamp DNA Mini Kit (Cat.: 51304 QIAGEN GmbH, Hilden, Germany), respectively. .. The RNA isolates of CVB3infected cells were subjected to one-step RT-qPCR amplification with Article Title: Integrated Phytochemical Profiling and Bioactivity Evaluation of Micromeria nervosa , with Emphasis on Antimicrobial and Antiviral Properties. Article Snippet: The DNA of HHV-1 and RNA of CVB3 were isolated using QIAamp DNA Mini Kit (Cat.: 51304 QIAGEN GmbH, Hilden, Germany) and QIAamp Viral RNA Mini Kit (Cat.: 52904 QIAGEN GmbH), respectively. .. The RNA isolates from CVB3-infected cells underwent one-step RT-qPCR amplification with Article Title: Genetic analysis of the X-linked adrenoleukodystrophy gene ABCD1 in Drosophila uncovers a conserved phenotype. Article Snippet: RNA was quantified using the DeNovix DS-11 FX Nanodrop (DeNovix, Wilmington, DE), following the manufacturer’s instructions. .. Real-time quantitative reverse transcription-PCR (real-time qRT-PCR) was performed to measure the mRNA levels of the studied genes using the CFX 96DX Touch real-time PCR detection system (Bio-Rad, Hercules, CA, USA). mRNA from 1 μg of total RNA was reverse transcribed into cDNA using the qScriptTM cDNA Synthesis Kit Cat. No. 95047-025 (Quanta BioSciences, Beverly, MA), following the manufacturer’s instructions. qRT-PCR amplification was performed using the Article Title: Vitamin D metabolites reshape bovine mammary epithelial responses relevant to mastitis through host-directed mechanisms Article Snippet: RNA quantity and quality were assessed with a microliter spectrophotometer (Picodrop, Hinxton, UK). .. RT-qPCR reactions were carried out using 100 ng of total RNA with the SYBR Green Assay:Article Title: Skeletons in the closet: The importance of actin in alphavirus replication Article Snippet: To measure Wolbachia titer, six-day-old mated female flies were snap-frozen in liquid nitrogen and stored at -80°C until genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen). qPCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad) on the CFX Opus 96 (Bio-Rad). .. To measure rhoGAP18B expression, six-day-old mated female flies were snap-frozen in liquid nitrogen and stored at -80°C until RNA was extracted using the RNeasy Mini Kit (Qiagen). qRT-PCR was performed using the Article Title: Local translation drives glioblastoma heterogeneity and tumor invasion Article Snippet: RNA quantity and integrity were assessed using an Agilent 4200 TapeStation with a High Sensitivity RNA ScreenTape (Agilent Technologies). .. Quantitative RT–PCR was performed using the Article Title: Extracts from the Edible Mushroom Sparassis crispa : Nematicidal, Antimicrobial, and Antiviral Properties Supporting Its Functional Food Potential. Article Snippet: The RNA of CVB3 and DNA of HSV-1 was isolated using QIAamp Viral RNA Mini Kit (Cat.: 52904 QIAGEN GmbH, Hilden, Germany) and QIAamp DNA Mini Kit (Cat.: 51304 QIAGEN GmbH, Hilden, Germany), respectively. .. The RNA isolates of CVB3infected cells were subjected to one-step RT-qPCR amplification with Article Title: Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation Article Snippet: Upon completion of the differentiation process, the 3D colonies were retrieved, and total RNA was extracted from the samples with a Direct-zol RNA MiniPrep Kit (Zymo Research Corp., Irvine, CA, USA) and diluted to 20 ng/μL. .. RT‒qPCR was conducted using the Bio-Rad CFX96TM TouchTM Real-time PCR Detection System and an Article Title: Integrated Phytochemical Profiling and Bioactivity Evaluation of Micromeria nervosa , with Emphasis on Antimicrobial and Antiviral Properties. Article Snippet: The DNA of HHV-1 and RNA of CVB3 were isolated using QIAamp DNA Mini Kit (Cat.: 51304 QIAGEN GmbH, Hilden, Germany) and QIAamp Viral RNA Mini Kit (Cat.: 52904 QIAGEN GmbH), respectively. .. The RNA isolates from CVB3-infected cells underwent one-step RT-qPCR amplification with Article Title: Genetic analysis of the X-linked adrenoleukodystrophy gene ABCD1 in Drosophila uncovers a conserved phenotype. Article Snippet: RNA was quantified using the DeNovix DS-11 FX Nanodrop (DeNovix, Wilmington, DE), following the manufacturer’s instructions. .. Real-time quantitative reverse transcription-PCR (real-time qRT-PCR) was performed to measure the mRNA levels of the studied genes using the CFX 96DX Touch real-time PCR detection system (Bio-Rad, Hercules, CA, USA). mRNA from 1 μg of total RNA was reverse transcribed into cDNA using the qScriptTM cDNA Synthesis Kit Cat. No. 95047-025 (Quanta BioSciences, Beverly, MA), following the manufacturer’s instructions. qRT-PCR amplification was performed using the Article Title: Vitamin D metabolites reshape bovine mammary epithelial responses relevant to mastitis through host-directed mechanisms Article Snippet: RNA quantity and quality were assessed with a microliter spectrophotometer (Picodrop, Hinxton, UK). .. RT-qPCR reactions were carried out using 100 ng of total RNA with the Article Title: Systemic Drivers and Molecular Mechanisms of Sarcopenia in Aetiology-Specific End-Stage Liver Disease. Article Snippet: .. mRNA levels of MAFbx, MuRF- 1, MYF5, IL- 6, MYOD, MYOG and FOXO were determined relative to the housekeeping gene 18S, using the Amplification:Article Title: Extracts from the Edible Mushroom Sparassis crispa : Nematicidal, Antimicrobial, and Antiviral Properties Supporting Its Functional Food Potential. Article Snippet: The RNA of CVB3 and DNA of HSV-1 was isolated using QIAamp Viral RNA Mini Kit (Cat.: 52904 QIAGEN GmbH, Hilden, Germany) and QIAamp DNA Mini Kit (Cat.: 51304 QIAGEN GmbH, Hilden, Germany), respectively. .. The RNA isolates of CVB3infected cells were subjected to one-step RT-qPCR amplification with Article Title: Integrated Phytochemical Profiling and Bioactivity Evaluation of Micromeria nervosa , with Emphasis on Antimicrobial and Antiviral Properties. 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No. 95047-025 (Quanta BioSciences, Beverly, MA), following the manufacturer’s instructions. qRT-PCR amplification was performed using the Real-time Polymerase Chain Reaction:Article Title: Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation Article Snippet: Upon completion of the differentiation process, the 3D colonies were retrieved, and total RNA was extracted from the samples with a Direct-zol RNA MiniPrep Kit (Zymo Research Corp., Irvine, CA, USA) and diluted to 20 ng/μL. .. RT‒qPCR was conducted using the Bio-Rad CFX96TM TouchTM Real-time PCR Detection System and an Article Title: Genetic analysis of the X-linked adrenoleukodystrophy gene ABCD1 in Drosophila uncovers a conserved phenotype. Article Snippet: RNA was quantified using the DeNovix DS-11 FX Nanodrop (DeNovix, Wilmington, DE), following the manufacturer’s instructions. .. Real-time quantitative reverse transcription-PCR (real-time qRT-PCR) was performed to measure the mRNA levels of the studied genes using the CFX 96DX Touch real-time PCR detection system (Bio-Rad, Hercules, CA, USA). mRNA from 1 μg of total RNA was reverse transcribed into cDNA using the qScriptTM cDNA Synthesis Kit Cat. No. 95047-025 (Quanta BioSciences, Beverly, MA), following the manufacturer’s instructions. qRT-PCR amplification was performed using the Article Title: Vitamin D metabolites reshape bovine mammary epithelial responses relevant to mastitis through host-directed mechanisms Article Snippet: RNA quantity and quality were assessed with a microliter spectrophotometer (Picodrop, Hinxton, UK). .. RT-qPCR reactions were carried out using 100 ng of total RNA with the Reverse Transcription:Article Title: Genetic analysis of the X-linked adrenoleukodystrophy gene ABCD1 in Drosophila uncovers a conserved phenotype. Article Snippet: RNA was quantified using the DeNovix DS-11 FX Nanodrop (DeNovix, Wilmington, DE), following the manufacturer’s instructions. .. Real-time quantitative reverse transcription-PCR (real-time qRT-PCR) was performed to measure the mRNA levels of the studied genes using the CFX 96DX Touch real-time PCR detection system (Bio-Rad, Hercules, CA, USA). mRNA from 1 μg of total RNA was reverse transcribed into cDNA using the qScriptTM cDNA Synthesis Kit Cat. No. 95047-025 (Quanta BioSciences, Beverly, MA), following the manufacturer’s instructions. qRT-PCR amplification was performed using the cDNA Synthesis:Article Title: Genetic analysis of the X-linked adrenoleukodystrophy gene ABCD1 in Drosophila uncovers a conserved phenotype. Article Snippet: RNA was quantified using the DeNovix DS-11 FX Nanodrop (DeNovix, Wilmington, DE), following the manufacturer’s instructions. .. Real-time quantitative reverse transcription-PCR (real-time qRT-PCR) was performed to measure the mRNA levels of the studied genes using the CFX 96DX Touch real-time PCR detection system (Bio-Rad, Hercules, CA, USA). mRNA from 1 μg of total RNA was reverse transcribed into cDNA using the qScriptTM cDNA Synthesis Kit Cat. No. 95047-025 (Quanta BioSciences, Beverly, MA), following the manufacturer’s instructions. qRT-PCR amplification was performed using the |